What Is Kcat Km. Web you will get vmax and km under your assay conditions’ used concentration of enzyme (e.g. To solve your question, (1)calculate kcat, i.e kcat=vmax/ [et] where [et]= total enzyme.
As km is constant, the affinity of the enzyme for the substrate should not change. On the other hand v max /2 is a velocity and is nothing more than that. Web the kcat /km ratio, where kcat is the catalytic constant for the conversion of substrate into product, and km is the michaelis constant, has been widely used as a measure of. Web you will get vmax and km under your assay conditions’ used concentration of enzyme (e.g. Web the kcat /k m value, or specificity constant, of the various substrates can be compared. Web k m is a dependent variable, and its value can change due to many reasons, including the ph level of the system, temperature, or any other condition. It is also the rate of catalyst with a. That substrate with the highest value is the best substrate for the enzyme, accounting for the name specificity constant. This is a good interpretation. The rate of any reaction is limited by the rate at which reactant.
The substrate concentration that gives you a rate that is halfway to v_ {max} v. Let suppose our vmax = 4mm/min calculation of kcat (the turnover. The substrate concentration that gives you a rate that is halfway to v_ {max} v. Web km is a substrate concentration and is the amount of substrate it takes for an enzyme to reach v max /2. It is also the rate of catalyst with a. Web the reciprocal form of the expression of kcat / km shows that kcat / km is a harmonic sum of kinetic terms that correspond to the heights of the transition states relative to. To solve your question, (1)calculate kcat, i.e kcat=vmax/ [et] where [et]= total enzyme. This is a good interpretation. Web kcat, or k2 or turnover number (they all mean the same thing) is a measure of how many substrates one (1) enzyme can convert into a product per second. Kcat/km is a useful measure of the efficiency. Web k m is a dependent variable, and its value can change due to many reasons, including the ph level of the system, temperature, or any other condition.
